AmpliTaq and AmpliTaq Gold DNA Polymerase
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چکیده
Nine Y-chromosome STRs were investigated in a male population sample from the Western Mediterranean region of Valencia (Eastern Spain). Complete nine Y-chromosomal STRs haplotypes were obtained in 140 individuals, among which 113 different haplotypes were observed. The most common haplotype was shared by 5% of the sample, while 99 haplotypes were unique. The gene diversity was 0.9892 and the discrimination capacity was 0.8071. Significant population differences were observed with respect to other Iberian populations, such as the Basques and Northern Portugueses.
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Enhancement of PCR amplification yield and specificity using AmpliTaq Gold DNA polymerase.
Inadequate yields of PCR product and the generation of nonspecific PCR products can complicate genotyping studies, particularly when the DNA template is of inferior quality and/or has a low-copy number. In this study, the ability of AmpliTaq Gold DNA Polymerase to enhance the specificity and yield of amplification was evaluated in a quadruplex short tandem repeat (STR) system in which a nonspec...
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To augment sensitivity and specificity of polymerase chain reaction (PCR), a nested PCR protocol is used (1). This procedure allows detection of even single copies of specific template sequences (5,6) but requires additional manipulations (such as opening of reaction tubes and transfer of PCR products), which may increase the risk of contamination and lead to false-positive results. To avoid th...
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Extensive diagnostic and scientific investigations are often restricted by limited availability of material. Therefore, methods like multiplex PCR strategies are needed to conserve as much sample as possible. Unfortunately, the establishment of such procedures poses several difficulties. Here we describe the advantages of a new enzyme, AmpliTaq Gold DNA Polymerase, in multiplex and time-release...
متن کاملAmpliTaq and AmpliTaq Gold DNA Polymerase
An, J. and A. W. Hsie (1993). "Polymerase chain reaction-based deletion screening of bleomycin induced 6-thioguanine-resistant mutants in Chinese hamster ovary cells: The effects of an inhibitor and a mimic of superoxide dismutase." Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 289(2): 215. http://www.sciencedirect.com/science/article/B6T2C-47PCN7M41/2/3157a48d39359d02b0...
متن کاملAmpliTaq and AmpliTaq Gold DNA Polymerase
An, J. and A. W. Hsie (1993). "Polymerase chain reaction-based deletion screening of bleomycin induced 6-thioguanine-resistant mutants in Chinese hamster ovary cells: The effects of an inhibitor and a mimic of superoxide dismutase." Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 289(2): 215. http://www.sciencedirect.com/science/article/B6T2C-47PCN7M41/2/3157a48d39359d02b0...
متن کاملAmpliTaq and AmpliTaq Gold DNA Polymerase
Oligodeoxyribonucleotide primers based on the 5' ends of bovine IgG1/2 and [lambda] constant (C) region genes, together with primers encoding conserved amino acids at the N-terminus of mature variable (V) regions from other species, have been used in cDNA and polymerase chain reactions (PCRs) to amplify heavy and light chain V region cDNA from bovine heterohybridomas. The amino acid sequences o...
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